biosynthesis of flavonoids

Abstract

Kalkon is one of a group of flavonoids that are very limited distribution in nature and is found only in a few groups of plants in small amounts. On the other hand, this kalkon compounds have useful biological activities, such as antioxidant, anti-inflammatory and antibacterial properties, so it needs an effort to get kalkon compounds by synthesis. From several studies suggest that group-containing compound kalkon methylenedioxy (-O-CH2-O-) have activity as an antibacterial, so in this study will be the synthesis of compounds derived kalkon with piperonal and acetophenone. Synthesis of 3,4-metilendioksikalkon through Claisen-Schmidt condensation reaction between piperonal and acetophenone using NaOH catalyst with various concentrations of NaOH catalyst is added at 40%, 50%, 60%, 70% and 80%. Results extracted using methylene chloride and then the solvent is evaporated using a rotary evaporator. The next stage characterization of physical properties of synthetic products and their structures analyzed using a spectrophotometer UV-Vis and FT-IR. After that tested antibacterial activity against bacteria E. coli and S. aureus. Compounds synthesized solid form, is yellow and has a melting point of 80-85 ° C. UV-Vis data converting the presence of two peaks, namely at λ 264 nm which is the absorption of benzoyl and λ 358 nm which is the absorption sinamoil. Data shows IR absorption spectra of the carbonyl group (C = O) at wavenumber 1658.7 cm-1, absorption C = C aromatic at wavenumber 1589.2 cm-1 and absorption at wavenumber 2923.9 cm-1 shows vibrational Csp3-H of methylene group (CH2). From these data it can be concluded that the compound 3,4-metilendioksikalkon been formed. Highest Yield of 72.58% was obtained at a concentration of 70% NaOH. From the results of the antibacterial test, the greatest inhibition zone occurs at concentrations of 3% which is 3 mm against E. coli and 2.5 mm against S. aureus.