NICOTINE

Tobacco production are abundant in Indonesia only useful as the tobacco industry are extremely negative impact on human health. Tobacco contains the alkaloid nicotine is bad for human health is also highly toxic to insects that nicotine can be used by humans As with the rice stem borer insecticide.

In this research, isolation of nicotine from tobacco leaves dried soxhletasi by using methanol and then do the salting with acid and alkaline extraction of alkaloids with. The extract obtained was then purified by TLC, and column chromatography. After it was analyzed using IR, UV, and GC-MS. Other tobacco extracts tested for its effectiveness as an insecticide rice stem borers by spraying the rice seedbed with various concentrations.

From the results of TLC analysis using prices obtained methanol solution developers Rf = 0.725. IR spectra analysis results indicate a tertiary aromatic amine, methyl, tertiary amine alifatis and aromatic CH bonds. The results of GC-MS chromatograms showed nicotine compounds appear at the top of the retention time = 9.245 s and 63% similarity index, this suggests that the tobacco alkaloid nicotine contained. Results from UV spectrophotometers generate maximum wavelength 206 nm indicating kearomatisan of pyridine ring in nicotine. Tobacco extracts are used to test the effectiveness of the rice stem borer, rice plants by spraying in which there is the rice stem borer, obtained Fhitung = 19,061 and F table = 3.48. Because Ftabel <Fhitung then Ho is rejected and Ha accepted, the concentration of tobacco leaf extracts affect its effectiveness as an insecticide rice stem borer.